NanoPlot report

Summary statistics

feature
General summary
Average percent identity	89.7
Mean read length	494.7
Mean read quality	9.1
Median percent identity	90.6
Median read length	353.0
Median read quality	9.0
Number of reads	649.0
Read length N50	656.0
Total bases	321,060.0
Total bases aligned	256,061.0
Number, percentage and megabases of reads above quality cutoffs
>Q5	649 (100.0%) 0.3Mb
>Q7	594 (91.5%) 0.3Mb
>Q10	209 (32.2%) 0.1Mb
>Q12	35 (5.4%) 0.0Mb
>Q15	0 (0.0%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1	14.3 (120; b85cfabd-f3fc-4e37-851f-243aa94869d8)
2	14.3 (113; 239374ac-fb65-45a2-af20-e75d19836af0)
3	14.1 (110; eb6a5fea-50e6-494e-bb17-4b40010f0063)
4	13.6 (305; b6473d0f-46e7-42cf-8148-36c069f869a6)
5	13.2 (107; b7c063a1-0ef4-4b6f-843a-b961f75ad631)
Top 5 longest reads and their mean basecall quality score
1	4774 (12.5; 61264d90-8f9f-46a3-ab32-554bb520cec6)
2	4509 (12.2; ed904c09-4b01-4c65-80be-d1e2defff27a)
3	2582 (10.8; 4b722f8e-d97d-4ed6-bec3-d8b0288ec3f3)
4	2470 (9.8; f3260f3e-7d90-4c4d-a284-461e6b6c92b8)
5	2047 (9.3; 2ea0fdcf-ae1d-43a8-a0b5-4c26584a4032)

Plots

Histogram of read lengths

Histogram of read lengths after log transformation

Weighted Histogram of read lengths

Weighted Histogram of read lengths after log transformation

sub2_norrna.sorted.bam

Yield by length

Read lengths vs Average read quality plot using hexagonal bins

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality plot using a kernel density estimation

Aligned read lengths vs Sequenced read length plot using hexagonal bins

Aligned read lengths vs Sequenced read length plot using dots

Aligned read lengths vs Sequenced read length plot using a kernel density estimation

Read mapping quality vs Average basecall quality plot using hexagonal bins

Read mapping quality vs Average basecall quality plot using dots

Read mapping quality vs Average basecall quality plot using a kernel density estimation

Read length vs Read mapping quality plot using hexagonal bins

Read length vs Read mapping quality plot using dots

Read length vs Read mapping quality plot using a kernel density estimation

Percent identity vs Average Base Quality plot using hexagonal bins

Percent identity vs Average Base Quality plot using dots

Percent identity vs Average Base Quality plot using a kernel density estimation

Aligned read length vs Percent identity plot using hexagonal bins

Aligned read length vs Percent identity plot using dots

Aligned read length vs Percent identity plot using a kernel density estimation

sub2_norrna.sorted.bam